Description
1. Fresh pancreatic tissue is mashed after removing lipids and connective tissue.
2. Remove nuclease and protease.
3. Soak the tissue residue in distilled water, mix, and extract.
4. (NH4)2SO4 precipitation to obtain Carboxypeptidase A.
5. Chromatographically further purify Carboxypeptidase A.
Enzyme Commission Number
EC 3.4.17.1
Activity
12-18 units/mg of protein
Unit Definition
1unit will hydrolyze 1µM of hippuryl-L-phenylalanine at pH 7.7 at 25°C.
Buffer
The substrate buffer is 0.025 M Tris pH 7.7, 0.1 M NaCl, 1 mM hippuryl-L-phenylalanine.
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