Description
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing hydrogen sulfide and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form 2-oxobutanoate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
homocysteine desulfurase; L-homocysteine hydrogen-sulfide-lyase (deaminating)
Reaction
L-homocysteine + H2O = hydrogen sulfide + NH3 + 2-oxobutanoate (overall reaction); (1a) L-homocysteine = hydrogen sulfide + 2-aminobut-2-enoate; (1b) 2-aminobut-2-enoate = 2-iminobutanoate (spontaneous); (1c) 2-iminobutanoate + H2O = 2-oxobutanoate + NH3 (spontaneous)
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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