Description
HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of HIV, the retrovirus that causes AIDS. HIV protease cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV protease, HIV virions remain uninfectious. Thus, mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells, making HIV protease inhibition the subject of considerable pharmaceutical research.
Abbr
HIV Protease, Recombinant
Package
100 unit vial containing > 100ug protein
Form
Supplied as a solution in 0.5M Sodium Acetate, pH 4.7with 50mM Tris-HCL, 1M NaCL, 1mM EDTA, 5mM DTT and 15% glycerol.
Enzyme Commission Number
EC 3.4.23.16
Molecular Weight
mol wt ~10.8 kDa
Unit Definition
One unit of HIV protease will cleave 1picomole per minute of the substrate Abz-Ala-Arg-Val-Nle-Tyr (NO2)-Glu-Ala-Nle-NH2 at 37°C.
Warnings
Bioengineered to resist autoproteolysis, resulting in an enzyme with improved stability and substantially higher activity levels over extended time periods to conduct kinetic assays. The enzyme includes the most commonlyeccurring background polymorphism that aids in the drug resistance profile.
Synonyms
human immunodeficiency virus type 1 protease; gag protease; HIV aspartyl protease; HIV proteinase; retroproteinase; HIV-1 protease; HIV-2 protease; HIV-1 retropepsin; HIV Protease Mutant; EC 3.4.23.16
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