Description
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
Abbr
HRS, Recombinant (Human)
Appearance
Sterile Filtered clear solution
Product Overview
Histidyl-tRNA Synthetase Human Recombinant produced in E. coli is a single, nonglycosylated, polypeptide chain having a molecular mass of 55 kDa. The Histidyl-tRNA Synthetase is purified by proprietary chromatographic techniques.
Enzyme Commission Number
EC 6.1.1.21
Purity
Greater than 90.0% as determined by both (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Stability
Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Buffer
The protein solution contains 150mM NaCl and 10mM sodium phosphate containing 0.1% NaN3 (pH 7.2).
Synonyms
Histidyl-tRNA synthetase; EC 6.1.1.21; Histidine-tRNA ligase; HisRS; HRS; FLJ20491; JO-1
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