Description
1. Porcine pancreatic tissue was physically broken and mixed with freezing buffer.
2. Decolorization.
3. Protease is separated and purified by centrifugation, electrophoresis and chromatography.
4. High-purity trypsin and chymotrypsin are obtained.
Activity
Trypsin:17.0-20.0 units/mg.
Chymotrypsin:16-19 units/mg.
Unit Definition
One unit hydrolyzes 1 µM of substrate per minute at 25°C.
Storage
Store 5°C. Stable 1-2 years.