Description
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate. Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.
Abbr
Heparinase I, Recombinant (Bacteroides eggerthii)
Species
Bacteroides eggerthii
Product Overview
Bacteroides Heparinase I cloned from Bacteroides eggerthii, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. In contrast to the Flavobacterium heparinum Heparinase I which cleaves the glycosidic bond between N-sulfated hexosamines and 2-O-sulfated iduronic acid residues, the Bacteroides Heparinase I cleaves between these same residues as well as the 2-O-sulfated glucuronic acid residues. The 2-O-sulfated uronic acid residue is essential for the activity of Bacteroides Heparinase I and 6-O-sulfation of GlcNS does not hinder enzyme activity. While Bacteroides Heparinase I cleaves 2-O sulfated iduronic acid and 2-O sulfated glucuronic acid residues at similar rates, the Flavobacterium heparinum Heparinase I has a much higher rate of cleavage for 2-O sulfated iduronic acid residues. Limited digest of porcine mucosal heparin with Flavobacterium heparinum Heparinase I results in sulfated heparin oligosaccharides structures previously reported. Limited digest of porcine mucosal heparin with the Bacteroides Heparinase I results in heparin oligosaccharides with a lower extent of sulfation as reported.
Form
100 mM NaCl, 20 mM Tris-HCl (pH 7.5 25°C), 1 mM Na2EDTA and 5 mM CaCl2.
Purity
> 95% determined by SDS-PAGE
Concentration
12,000 units/ml
Unit Definition
One unit is defined as the amount of enzyme that will liberate 1.0 μmol unsaturated oligosaccharides from porcine mucosal heparin per minute at 30°C and pH 7.0 in a total reaction volume of 100 μl.
Synonyms
Heparinase; Heparin lyase; Heparin eliminase; Heparin-sulfate lyase; Heparin-sulfate eliminase; Heparitin-sulfate lyase; Heparinase I; Heparinase III; Heparin lyase II; Heparinase II
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