Description
G/U mismatch-specific DNA glycosylase (mug) is a part of the TDG/mug DNA glycosylase family. Mug is necessary for DNA damage lesion repair in stationary-phase cells. Mug protein removes three N4-ethenocytosine and takes away s the uracil base from mismatches in the order of U:G>U:A. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP position. Mug is also able to hydrolyzing the carbon-nitrogen bond among the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine plays a role in substrate recognition.
Abbr
MUG from E.coli, Recombinant
Form
Sterile Filtered colorless solution.
Enzyme Commission Number
EC 3.2.2.28
Molecular Weight
21.1 kDa
Purity
Greater than 90% as determined by SDS-PAGE.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Synonyms
Xanthine DNA glycosylase; dug; ECK3058; JW3040; ygjF; G/U mismatch-specific DNA glycosylase; Double-strand-specific uracil glycosylase; Mismatch-specific uracil DNA-glycosylase; mug
Formulation
The MUG solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 20% glycerol.
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