Products
Online Inquiry
Our Products Cannot Be Used As Medicines Directly For Personal Use.
Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.
Green 500 dUTP
Cat No.
CSUB-0816
Description
Green 500 [5(6)-Carboxyrhodamine Green] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Green 500 dUTP can also be used for multicolor fluorescence labeling.
Applications
Green 500 [5(6)-Carboxyrhodamine Green] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
Form
Liquid
Synonyms
5(6)-Carboxyrhodamine Green dUTP
Solubility
Soluble in water (fully).
Substrates
Fucosyltransferase
Refractive Index
1.87 (Predicted)
Download
Download Datasheet