Description
The glyoxalase system consists of Glyoxalase I (Glx I) and Glyoxalase II (Glx II) which is responsible for detoxifying α-ketoaldehydes such as the potent and cytotoxic methylglyoxal (MG). Glx I converts MG to S-D-lactoyl-glutathione (SLG). SLG is then hydrolyzed to D-lactate and reduced glutathione by Glx II. Glx II desensitizes cells to p53-dependent DNA damage-induced apoptosis. It also plays a role during development and in the pathogenesis of cancer and neurodegenerative diseases. Glyoxalase II may also be important in the regulation of spermatogenesis.
Abbr
Glx II, Recombinant (Human)
Product Overview
Glx II amino acid 14-308 (295 amino acids) with a C-terminal 6x His-tag.
Enzyme Commission Number
EC 3.1.2.6
Molecular Weight
32.4 kDa
Unit Definition
One unit is the amount of enzyme that will hydrolyze 1.0 µmole of SLG per minute at pH 7.4 at 25°C.
Storage
Store at -20 ºC for at least two years. Avoid repeated freeze thaw cycles.
Synonyms
Hydroxyacylglutathione hydrolase; mitochondrial; HAGH1; GLO2
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