Description
Glycogen phosphorylase is one of the phosphorylaseenzymes (EC 2.4.1.1). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule, and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism, it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase. Glycogen phosphorylase can only act on linearchainsof glycogen (a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch (which are exceedingly common in glycogen). In these situations, a debranching enzymeis necessary, which will straighten out the chain in that area. Additionally, an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done, glycogen phosphorylase can continue.
Abbr
GPBB, Recombinant (Human)
Applications
Immunoassays and western blot.
Appearance
Sterile Filtered colourless liquid formualtion.
Product Overview
Glycogen Phosphorylase Human Recombinant produced in E. coli is a single, nonglycosylated, polypeptide chain. The Human GPBB mature chain: 2-843 aa; that is a total of 842 aa having a molecular mass of 96695.96 Dalton. The theoretical pI is 6.40. The GPBB is purified by proprietary chromatographic techniques.
Purity
Greater than 85.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Stability
GPBB although stable at 10°C for 7 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Buffer
0.8 mg/1ml, each mg of protein contains 50% glycerol.
Synonyms
glycogen phosphorylase; muscle phosphorylase a and b; amylophosphorylase; polyphosphorylase; amylopectin phosphorylase; glucan phosphorylase; α-glucan phosphorylase; 1,4-α-glucan phosphorylase; glucosan phosphorylase; granulose phosphorylase; maltodextrin phosphorylase; muscle phosphorylase; myophosphorylase; potato phosphorylase; starch phosphorylase; 1,4-α-D-glucan:phosphate α-D-glucosyltransferase; phosphorylase; EC 2.4.1.1; GPBB
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