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Glycerol-3-Phosphate Dehydrogenase from E. coli, Recombinant
Cat No.
NATE-1904
Description
α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate.
Abbr
G3PDH, Recombinant (E. coli)
Alias
G3PDH; α-GDH
Source
E. coli
Applications
The enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerokinase.
Appearance
Lyophilized
Enzyme Commission Number
EC 1.1.1.8
CAS No.
9075-65-4
Molecular Weight
ca. 73,600; Subunit molecular weight : ca. 36,800.
pH Stability
6.5 - 10.0
Michaelis Constant
(90 mM Bicine buffer pH 9.0, at 37 °C)
Glycerol-3-phosphate: 0.119 mM;
NAD+: 0.036 mM.
Glycerol-3-phosphate: 0.119 mM;
NAD+: 0.036 mM.
Unit Definition
One unit of activity is defined as the amount of G3PDH that forms 1 μmol of NADH per minute at 37 °C.
Optimum pH
9
Thermal stability
No detectable decrease in activity up to 80 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
α-glycerol phosphate dehydrogenase (NAD); α-glycerophosphate dehydrogenase (NAD); glycerol 1-phosphate dehydrogenase; glycerol phosphate dehydrogenase (NAD); glycerophosphate dehydrogenase (NAD); hydroglycerophosphate dehydrogenase; L-α-glycerol phosphate dehydrogenase; L-α-glycerophosphate dehydrogenase; L-glycerol phosphate dehydrogenase; L-glycerophosphate dehydrogenase; NAD-α-glycerophosphate dehydrogenase; NAD-dependent glycerol phosphate dehydrogenase; NAD-dependent glycerol-3-phosphate dehydrogenase; NAD-L-glycerol-3-phosphate dehydrogenase; NAD-linked glycerol 3-phosphate dehydrogenase; NADH-dihydroxyacetone phosphate reductase; glycerol-3-phosphate dehydrogenase (NAD); EC 1.1.1.8; 9075-65-4; α-GDH
Specific Activity
more than 7 U/mg protein
Reaction
Glycerol-3-phosphate + NAD+ ←→ Dihydroxyacetone 3-phosphate + NADH+ H+
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0313 | Native Rabbit Glycerol-3-phosphate dehydrogenase | EC 1.1.1.8 | 9075-65-4 | Rabbit Muscle | Inquiry |
| NATE-0750 | Native Rabbit α-Glycerophosphate Dehydrogenase | EC 1.1.1.8 | 9075-65-4 | Rabbit muscle | Inquiry |
| NATE-0751 | Native Rabbit α-Glycerophosphate Dehydrogenase-Triosephosphate Isomerase | EC 1.1.1.8 | 9075-65-4 | Rabbit muscle | Inquiry |
| EXWM-0363 | glycerol-3-phosphate dehydrogenase (NAD+) | EC 1.1.1.8 | 9075-65-4 | Inquiry |