Description
α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate.
Abbr
G3PDH, Recombinant (E. coli)
Applications
The enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerokinase.
Enzyme Commission Number
EC 1.1.1.8
Molecular Weight
ca. 73,600; Subunit molecular weight : ca. 36,800.
Michaelis Constant
(90 mM Bicine buffer pH 9.0, at 37 °C)
Glycerol-3-phosphate: 0.119 mM;
NAD+: 0.036 mM.
Unit Definition
One unit of activity is defined as the amount of G3PDH that forms 1 μmol of NADH per minute at 37 °C.
Thermal stability
No detectable decrease in activity up to 80 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
α-glycerol phosphate dehydrogenase (NAD); α-glycerophosphate dehydrogenase (NAD); glycerol 1-phosphate dehydrogenase; glycerol phosphate dehydrogenase (NAD); glycerophosphate dehydrogenase (NAD); hydroglycerophosphate dehydrogenase; L-α-glycerol phosphate dehydrogenase; L-α-glycerophosphate dehydrogenase; L-glycerol phosphate dehydrogenase; L-glycerophosphate dehydrogenase; NAD-α-glycerophosphate dehydrogenase; NAD-dependent glycerol phosphate dehydrogenase; NAD-dependent glycerol-3-phosphate dehydrogenase; NAD-L-glycerol-3-phosphate dehydrogenase; NAD-linked glycerol 3-phosphate dehydrogenase; NADH-dihydroxyacetone phosphate reductase; glycerol-3-phosphate dehydrogenase (NAD); EC 1.1.1.8; 9075-65-4; α-GDH
Specific Activity
more than 7 U/mg protein
Reaction
Glycerol-3-phosphate + NAD+ ←→ Dihydroxyacetone 3-phosphate + NADH+ H+
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