Description
The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases, e.g. the tetrasaccharide β-D-4-deoxy-Δ4-GlcAp-(1→4)-β-D-Glcp-(1→4)-α-L-Rhap-(1→3)-β-D-Glcp produced by EC 4.2.2.25, gellan lyase. The enzyme can also hydrolyse unsaturated chondroitin and hyaluronate disaccharides (β-D-4-deoxy-Δ4-GlcAp-(1→3)-β-D-GalNAc, β-D-4-deoxy-Δ4-GlcAp-(1→3)-β-D-GalNAc6S, β-D-4-deoxy-Δ4-GlcAp2S-(1→3)-β-D-GalNAc, β-D-4-deoxy-Δ4-GlcAp-(1→3)-β-D-GlcNAc), preferring the unsulfated disaccharides to the sulfated disaccharides.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
UGL (ambiguous); unsaturated glucuronyl hydrolase (ambiguous)
Reaction
β-D-4-deoxy-Δ4-GlcAp-(1→4)-β-D-Glcp-(1→4)-α-L-Rhap-(1→3)-β-D-Glcp + H2O =5-dehydro-4-deoxy-D-glucuronate + β-D-Glcp-(1→4)-α-L-Rhap-(1→3)-β-D-Glcp
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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