Description
Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells. Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It has a molecular mass of 52.7 kDa. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells. Furin cleaves precursor proteins at their paired basic amino acid processing sites. Some substrates of furin include von Willebrand factor, transforming growth factor beta 1 precursor, pro-beta-secretase and proparathyroid hormone.
Abbr
Furin, Recombinant (Human)
Source
Baculovirus infected Sf9 cells
Applications
Furin is capable of cleaving precursors of a wide variety of proteins, including growth factors, serum proteins, including proteases of the blood-clotting and complement systems, matrix metalloproteinases, receptors, viral-envelope glycoproteins, and bacterial exotoxins, typically at sites marked by the consensus sequence Arg-Xaa-(Lys/Arg)-Arg.
Product Overview
recombinant, expressed in baculovirus infected Sf9 cells
Form
buffered aqueous solution
Unit Definition
One unit is defined as the amount of Furin that will release 1 pmole of AMC from the fluorogenic peptide Boc-RVRR-AMC in 1 minute at 30°C.
Buffer
Solution in 10 mM MES, pH 7.0 at 25°C, 1 mM CaCl2, 50% glycerol.
Pathway
Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Gamma-carboxylation, transport, and amino-terminal cleavage of proteins, organism-specific biosystem; Glypican 3 network, organism-specific biosystem
Function
endopeptidase activity; metal ion binding; nerve growth factor binding; peptidase activity; peptide binding; protease binding; serine-type endopeptidase activity; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity
Synonyms
furin; prohormone convertase; dibasic processing enzyme; PACE; paired basic amino acid cleaving enzyme; paired basic amino acid converting enzyme; serine proteinase PACE; PC1; SPC3; proprotein convertase
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