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Free Methionine-(R)-Sulfoxide Reductase from E. coli, recombinant
Cat No.
NATE-1693
Description
The Free Methionine-(R)-Sulfoxide Reductase (fRMsr) reduces free methionine sulfoxide (Met(O)) to methionine using thiol-disulfide exchange chemistry. This enzyme is involved in oxidative defense and known to form a sulfenic acid intermediate at the active site Cys during the course of turnover. In this variant, all Cys other than the peroxide-sensitive Cys have been removed by mutagenesis in order to stabilize the active site sulfenic acid with respect to disulfide bond formation.
Abbr
fRMsr, Recombinant (E. coli)
Source
E. coli
Applications
Free Methionine-(R)-Sulfoxide Reductase (C84S, C94S), or fRMsr, can be selectively derivatized at a single Cys residue with a variety of Cys-SOH specific probes and be used as a positive control.
Form
Liquid
Enzyme Commission Number
EC 1.8.4.14
Molecular Weight
18,752 Da
Purity
>98% by SDS-PAGE
Concentration
10mg/mL
pH Stability
5.5-8.0
Storage
at -80 °C
Buffer
20mM Hepes pH 7.5, 100mM NaCl
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