Description
The Free Methionine-(R)-Sulfoxide Reductase (fRMsr) reduces free methionine sulfoxide (Met(O)) to methionine using thiol-disulfide exchange chemistry. This enzyme is involved in oxidative defense and known to form a sulfenic acid intermediate at the active site Cys during the course of turnover. In this variant, all Cys other than the peroxide-sensitive Cys have been removed by mutagenesis in order to stabilize the active site sulfenic acid with respect to disulfide bond formation.
Abbr
fRMsr, Recombinant (E. coli)
Applications
Free Methionine-(R)-Sulfoxide Reductase (C84S, C94S), or fRMsr, can be selectively derivatized at a single Cys residue with a variety of Cys-SOH specific probes and be used as a positive control.
Enzyme Commission Number
EC 1.8.4.14
Molecular Weight
18,752 Da
Buffer
20mM Hepes pH 7.5, 100mM NaCl
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