Product Overview
Fagomine is a good inhibitor of various Glycosidases, including rat intestinal surcase and bovine α-Fucosidase with IC50 of 90μM and 140 μM, respectively. Fagomine has been shown to have some activity against mammalian gut R-glucosidase and β-galactosidase, Moreover, fagomine was also found to have a potent antihyperglycemic effect in streptozocin-induced diabetic mice and to potentiate markedly immunoreactive insulin release. Fagomine inhibitory activity was tested against different enzymes with IC50 of 90μM and 140 μM for rat intestinal surcase and bovine α-Fucosidase, respectively. Furthermore, the IC50 values for α-glucosidase in rice and yeast, along with rat intestinal maltase and isomaltase were also determined and the results were 320 880 820 and 460μM, respectively. The activities of rice R-glucosidase and rat digestive glycosidases were determined using the appropriate disaccharides as substrates at the optimum pH of each enzyme. The released D-glucose was determined colorimetrically using the glucose B-test. Other glycosidase activities were determined using an appropriate p-nitrophenyl glycoside as a substrate at the optimum pH of each enzyme. D-Fagomine lowered postprandial glycaemia in SD rats after the intake of sucrose and starch. D-Fagomine effectively regulated postprandial glycaemia in a dose-dependent manner, which led to a reduction in AUC at 120 min between 17 % and 43 % after the intake of 1 g sucrose/kg body weight. D-Fagomine effectively modulates the glucose level in blood in a dose-dependent manner after the ingestion of sucrose or starch, probably by delaying saccharide hydrolysis by brush-border glycosidases.
Chemical Name
(2R,3R,4R)-2-(hydroxymethyl)piperidine-3,4-diol
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