Description
In enzymology, a pectate disaccharide-lyase (EC 4.2.2.9) is an enzyme that catalyzes the chemical reaction: Eliminative cleavage of 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate from the reducing end of pectate, i.e. de-esterified pectin. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides.
Abbr
Exo-pectate lyase, Recombinant (Dickeya dadantii)
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 4.2.2.9
Molecular Weight
65.0 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
Polygalacturonans or short oligogalacturonans
Optimum temperature
37 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
pectate disaccharide-lyase; EC 4.2.2.9; (1->4)-alpha-D-galacturonan reducing-end-disaccharide-lyase; pectate exo-lyase; exopectic acid transeliminase; exopectate lyase; exopolygalacturonic acid-trans-eliminase; PATE; exo-PATE; exo-PGL
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