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Exonuclease III
Cat No.
NATZ-080
Description
1. Contains DNA 3'-phosphatase: hydrolyzes 3'-terminal phosphate monoesters and can hydrolyze hydrolyzed DNA from the 3'-end.
2. Contains AP endonuclease: cleaves the phosphodiester bond at the purine or pyrimidine site, generating a base-free deoxyribose 5'-phosphate residue at the 5'-end of the residue.
3. Ribonuclease H activity: degrades RNA strands in DNA-RNA hybrid double strands.
4. Digests double-stranded DNA at the gap in the single-stranded interstices.
5. Will not degrade double-stranded DNA, single-stranded DNA, or phosphorothioate-conjugated nucleotides with at least 4 base pair overhangs.
2. Contains AP endonuclease: cleaves the phosphodiester bond at the purine or pyrimidine site, generating a base-free deoxyribose 5'-phosphate residue at the 5'-end of the residue.
3. Ribonuclease H activity: degrades RNA strands in DNA-RNA hybrid double strands.
4. Digests double-stranded DNA at the gap in the single-stranded interstices.
5. Will not degrade double-stranded DNA, single-stranded DNA, or phosphorothioate-conjugated nucleotides with at least 4 base pair overhangs.
Source
E.coli
Form
Liquid
Enzyme Commission Number
EC 3.1.11.2
Purity
≥90%
Storage
-20℃
Buffer
10X Exonuclease III Reaction Buffer
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