Description
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor.
Abbr
TG 2, Recombinant (Human)
Applications
Transglutaminase 2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. Transglutaminase 2 may also be used for immunoprecipitation. This product is suitable for cell culture use.
Form
The transglutaminase is supplied in 10 mM Tris-HCl pH 7.2, 150 mM NaCl, 0.5 mM EDTA, 0.5 mM DTT, 10% Glycerol.
Enzyme Commission Number
EC 2.3.2.13
Activity
> 1500 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after transglutaminase-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).
Unit Definition
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Activators
Add 10 mM Ca2+ to activate transglutaminase.
Storage
Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Synonyms
transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase; TG1
Endotoxin Level
Endotoxin level: < 4.5 EU/mg, sterile filtered using 0.2 micron filter [Bacterial endotoxin level was determined at SGS Institute Fresenius using the kinetic - turbidimetric method].
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