Description
In enzymology, an endoglycosylceramidase (EC 3.2.1.123) is an enzyme that catalyzes the chemical reaction:oligoglycosylglucosylceramide + H2O↔ ceramide + oligoglycosylglucose. Thus, the two substrates of this enzyme are oligoglycosylglucosylceramide and H2O, whereas its two products are ceramide and oligoglycosylglucose. This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O-and S-glycosyl compounds.
Abbr
EGCase, Recombinant (Rhodococcus sp.)
Applications
Endoglycoceramidase II from Rhod oc occus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry. Endoglycoceramidase II from Rhodococcus sp. has also been used in a study to investigate structural and mechanistic analyses of Endoglycoceramidase II.
Form
Solution in 20 mM sodium acetate buffer, pH 6.0, containing 0.2% BSA and 0.1% Lubrol PX.
Enzyme Commission Number
EC 3.2.1.123
Unit Definition
One unit will hydrolyze 1 μmol of asialo-GM1 per min at 37°C at pH 5.0.
Synonyms
EC 3.2.1.123, endoglycoceramidase; EGCase; glycosyl-N-acetyl-sphingosine 1,1-β-D-glucanohydrolase, oligoglycosylglucosylceramide glycohydrolase; oligoglycosylglucosyl (1↔1)ceramide glycohydrolase
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