Description
Arabinan endo-1,5-alpha-L-arabinanase is an enzyme with system name 5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase. This enzyme catalyses the following chemical reaction: Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans. This enzyme is most active on linear 1,5-alpha-L-arabinan.
Abbr
Endo-arabinanase, Recombinant (Streptomyces coelicolor)
Source
Streptomyces coelicolor
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.99
Molecular Weight
33858.2 Da
Purity
> 80 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of D-glucose equivalents per minute from sugar beet arabinan (4.00 mg/mL) in 50 mM sodium acetate buffer, pH 6.0, containing 1.0 mg/mL BSA, at 35°C, and using the DNSA assay method of Miller to follow reducing sugar liberated at 575 nm.
Optimum temperature
> 35°C
Storage
Store at 4°C (shipped at room temperature)
Synonyms
EC 3.2.1.99; endo-exo-Arabinanase; endo-1,5-alpha-L-arabinanase; endo-alpha-1,5-arabanase; endo-arabanase; 1,5-alpha-L-arabinan 1,5-alpha-L arabinanohydrolase
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