Description
The enzymes from the archaea Hyperthermus butylicus and Sulfophobococcus zilligii are active with ATP, ADP or GTP. They show no activity with NAD+. Different from EC 6.5.1.1, DNA ligase (ATP), which is specific for ATP, and EC 6.5.1.6, DNA ligase (ATP or NAD+) which can utilize either ATP or NAD+.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Reaction
(1) ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + diphosphate + (deoxyribonucleotide)n+m; (2) ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = AMP + phosphate + (deoxyribonucleotide)n+m; (3) GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m = GMP + diphosphate + (deoxyribonucleotide)n+m
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Download Datasheet