Description
The diguanylate cyclase from Agrobacterium vitis has been engineered to remove phosphodiesterase activity, allowing for production of cyclic-diGMP from guanosine triphosphate (GTP) without the production of 5'-phosphoguanylyl-(3',5')-guanosine (pGpG).
Abbr
DGC, Recombinant (Agrobacterium vitis)
Species
Agrobacterium vitis
Applications
Useful for producing cyclic-diGMP from GTP without production of pGpG
Completely lacks unwanted phosphodiesterase activity
No product inhibition even at high concentrations of GTP
Remains active while immobilized to solid resin and retain enzymatic activity after several months of storage
Can be used to synthesize radiolabeled cyclic diGMP from radiolabeled GTP
Enzyme Commission Number
EC 2.7.7.65
Purity
>99% based on SDS-PAGE analysis with coomassie blue
Unit Definition
One unit (U) is 1 µmole of H2 evolved min-1 mg-1.
Thermal stability
Reactions can be run at 25 to 37 °C with 50 mM Tris HCl, pH 7.5 containing 5 mM MgCl2 as the buffer
Storage
at -80 °C; Multiple freeze/thaw cycles are not recommended
Buffer
50 mM Tris-HCl, pH 7.4, 5 mM β-mercaptoethanol, 10% glycerol, 50 mM arginine, 50 mM glutamic acid, 200 mM sodium chloride, 500 uM ethylenediaminetetraacetic acid (EDTA) and 10% glycerol.
Synonyms
DGC; PleD; EC 2.7.7.65; 146316-82-7; Engineered Diguanylate Cyclase
Formulation
0.1 mg/ml (100 U/µl) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0
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