Abbr
dCK, Recombinant (Human)
Applications
Human deoxycytidine kinase is an active and purified protein of 33kDa cloned by RT-PCR amplification of mRNA extracted from human hepatoma cells and expressed in E.coli.
Package
stable lyophilized form
Enzyme Commission Number
EC 2.7.1.74
Activity
≥ 0.025 unit/mg protein
Unit Definition
One unit of deoxycytidine kinase converts 1.0 µmole of deoxyinosine and ATP to dCMP and ADP per minute at pH 7.6 at 37°C, as measured by a coupled PK/LDH enzyme system.
Storage
–20 °C in a solution containing 50 mM Tris-HCl, pH 7.6, 1 mM β-mercaptoethanol, 50% glycerol.
Synonyms
deoxycytidine kinase (phosphorylating); 2'-deoxycytidine kinase; Ara-C kinase; arabinofuranosylcytosine kinase; deoxycytidine-cytidine kinase; dCK
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