Description
Deoxycytidine kinase (dCK, EC:2.7.1.74) is required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG), and deoxyadenosine (dA). dCK has a broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
Abbr
DCK, Recombinant (Human)
Enzyme Commission Number
EC 2.7.1.74
Activity
145 IU/mg protein
Unit Definition
One unit of WT human dCK converts 1.0 µmole of dC and ATP to dCMP and ADP per minute at pH 7.5 at 37°C, as measured by a coupled enzyme system with 200 µM dC and 1 mM ATP.
Buffer
25 mM Tris pH7.5, 500 mM NaCl, 20 % glycerol, 10 mM DTT, 1 mM EDTA.
Synonyms
Human Dck; dCK-DM
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