Description
D-2-hydroxyglutarate (D2HG) level is significantly increased in metabolic diseases and various cancers such as acute myeloid leukemia. Studies suggest that the detection of D2HG serves as a biomarker assay related to IDH (isocitrate dehydrogenase) mutations. D2HGDH is a special NAD-dependent enzyme which reacts with D2HG specifically and converts D2HG to α-ketoglutarate. D2HGDH is a key enzyme to distinguish between two metabolites, D2HG and L-2-hydroxyglutarate (L2HG), during biomarker assays.
Abbr
D2HGDH, Recombinant (Acidaminococcus fermentans)
Species
Acidaminococcus fermentans
Form
Lyophilized in 50mM Tris, pH 8 without any additives.
Enzyme Commission Number
EC 1.1.99.39
Purity
> 99% by SDS - PAGE
Unit Definition
One unit is the amount of enzyme that reduces 1.0 µmole of NAD+ to NADH per min. at pH 8 at 37°C.)
Storage
Can be stored at 4°C up to 2 weeks. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
Synonyms
D-2-hydroxyglutarate dehydrogenase; D2HGDH; D2HGD
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-5 mg/ml. The solution can be diluted into other aqueous buffers and stored at –20°C for future use.
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