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cysteine-S-conjugate β-lyase
Cat No.
EXWM-5315
Description
A pyridoxal-phosphate protein. The enzyme can act on a broad range of L-cysteine-S-conjugates, including aromatic conjugates such as 4-bromobenzene and 2,4-dinitrobenzene. The enzyme cleaves a carbon-sulfur bond, releasing a thiol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase.
Form
Liquid or lyophilized powder
Enzyme Commission Number
CAS No.
68652-57-3
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
cysteine conjugate β-lyase; glutamine transaminase K/cysteine conjugate β-lyase; L-cysteine-S-conjugate thiol-lyase (deaminating)
Reaction
an L-cysteine-S-conjugate + H2O = a thiol + NH3 + pyruvate (overall reaction); (1a) an L-cysteine-S-conjugate = a thiol + 2-aminoprop-2-enoate; (1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous); (1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous);
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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