Description
Cysteine protease is an enzyme for single site digestion of antibodies in the hinge region. Incubation for one hour at 37°C under reducing conditions is enough for cysteine protease to cleave all subclasses of human, mouse, rat, goat and sheep IgG. Cysteine protease is supplied as lyophilized powder with no preservatives added and is available in a 2000 units pack size. Cysteine protease contains a His-tag and can easily be removed after digestion.
Abbr
Cysteine protease, Recombinant (Streptocoocus pyogenes)
Species
Streptocoocus pyogenes
Appearance
White to light yellow powder
Form
Lyophilized powder with no preservatives added
Purity
> 95% homogeneity as determined by SDS-PAGE analysis using Coomassie Blue detection.
Unit Definition
One unit digests > 95% of 1µg human or mouse IgG when incubated in 10 mM Tris, 150 mM NaCl pH 7.4 at 37°C for 1 hour.
Stability
The enzyme is reconstituted by addition of water and after reconstitution it is stable for 1 month at +4-8°C. The product is shipped on ice and should be stored at -20°C upon arrival.
Buffer
Cysteine protease cleaves IgG in commonly used buffers with pH ranging from 6.5 to 8.0 and best activity is obtained at 37°C. The enzyme requires reducing agent in the digestion reaction and depending on the reducing conditions there is a chance that the interchain thiols will be reduced.
Synonyms
Cysteine protease; FabULOUS
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