Description
Cyclooxygenase 2 (COX-2) catalyzes the first step in the biosynthesis of prostaglandins (PGs), thromboxanes, and prostacyclins: The conversion fo arachidonic acid to PGH2. Discoveries of the induction of COX expression by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of COX, COX-2, is responsible for the biosynthesis of PGs under acute inflammatory conditions. Thus, COX-2 has become the focus of attention for the nonsteroidal anti-inflammatory drug (NSAID) development. Human recombinant COX-2 contains a six residue histidine sequence (His-tag) near the amino terminus. The His-tag enzyme, which has a Km value for arachidonate of 6.5 µM, exhibits enzyme activity and sensitivity to NSAIDs similar to the non-tagged enzyme.
Abbr
COX-2, Recombinant (Ovine)
Purity
>80% estimated by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to consume 1 nmol of oxygen per minute 37°C in 100 mM Tris, pH 8.0, containing 100 µm arachidonate, 5 mM EDTA, 2 mM phenol, and 1 µM hematin.
Storage
-80°C (as supplied)
Synonyms
Cyclooxygenase 2; Inducible Cyclooxygenase Prostaglandin H Synthase 2; COX-2
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