Description
The enzyme contains three functionally distinct sites: an allosteric GTP-binding site, a glutaminase site where glutamine hydrolysis occurs (cf. EC 3.5.1.2, glutaminase), and the active site where CTP synthesis takes place. The reaction proceeds via phosphorylation of UTP by ATP to give an activated intermediate 4-phosphoryl UTP and ADP. Ammonia then reacts with this intermediate generating CTP and a phosphate. The enzyme can also use ammonia from the surrounding solution.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
UTP-ammonia ligase; cytidine triphosphate synthetase; uridine triphosphate aminase; cytidine 5'-triphosphate synthetase; CTPS (gene name); pyrG (gene name); CTP synthase; UTP:ammonia ligase (ADP-forming)
Reaction
ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate (overall reaction); (1a) L-glutamine + H2O = L-glutamate + NH3; (1b) ATP + UTP + NH3 = ADP + phosphate + CTP
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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