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Collagen Type II from Bovine Nasal
Cat No.
CEFX-020
Description
1. Extract the tissue with guanidine-HCl to remove most of the proteoglycans.
2. Collagen was dissolved with pepsinized acetic acid at pH 3.0.
3. Fractional precipitation with NaCl and dialysis precipitation with Na2HPO4.
4. Column chromatography on CM-cellulose at pH 4.0.
5. Column chromatography on DEAE-cellulose at pH 7.5 to remove trace amounts of residual proteoglycans and collagen types I and III.
The proteoglycan content is less than 0.4%. Soluble in 0.01 M to 0.5 M HOAC.
2. Collagen was dissolved with pepsinized acetic acid at pH 3.0.
3. Fractional precipitation with NaCl and dialysis precipitation with Na2HPO4.
4. Column chromatography on CM-cellulose at pH 4.0.
5. Column chromatography on DEAE-cellulose at pH 7.5 to remove trace amounts of residual proteoglycans and collagen types I and III.
The proteoglycan content is less than 0.4%. Soluble in 0.01 M to 0.5 M HOAC.
Source
Bovine Nasal
Species
Bovine
Form
Powder
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