Description
1. Thoroughly wash skins in cold water .
2. Grind clean skins at low temperature.
3. Stir the ground tissue with 0.5 M pre-cooled sodium acetate solution for 15-18 hours, then centrifuge to remove the supernatant (repeat the procedure four times).
4. the residue is washed with distilled water (three times).
5. extract with 0.075 M citrate buffer at pH 3.7 and centrifuge to obtain supernatant.
6. dialyze with 0.02 M disodium hydrogen phosphate solution.
7. wash with cold distilled water, resuspend, freeze-dry.
Soluble in 0.01 M to 0.5 M HAc and acidic buffers; Maximum of 5 mg/ml.
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