Description
Chondroitinase ABC cleaves, via an elimination mechanism, sulfated and non-sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme is active on chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid.
Applications
Determination of contents of chondroitin sulfates by HPLC
Processing animal tissues before further investigations
Preparation of chondroitin and hyaluronic acid derived unsaturated disaccharides
Preparation of hyaluronic acid derived unsaturated disaccharide
Enzyme Commission Number
EC 4.2.2.20
Activity
> 100 IU/mg (substrate: chondroitin sulfate A).
Molecular Weight
117.3 kDa
Specificity
Chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid.
Unit Definition
One international unit (IU) of chondroitinase ABC is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from chondroitin sulfates A per minute at 30 °C and pH 8.0.
Optimum temperature
37 °C
Stability
Expiration is at least 12 months from manufacturing date, kept at -20 °C.
Synonyms
EC 4.2.2.4, chondroitinase; chondroitin ABC eliminase; chondroitinase ABC; chondroitin ABC lyase; chondroitin sulfate ABC lyase; ChS ABC lyase; chondroitin sulfate ABC endoeliminase; chondroitin sulfate ABC endolyase; ChS ABC lyase I; 9024-13-9
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