Description
This enzyme, found in several bacterial pathogens, is involved in degradation of the host cholesterol. It catalyses the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol. The products are exclusively in the (25R) conformation. The enzyme also accepts cholesterol as a substrate. cf. EC 1.14.13.141, cholest-4-en-3-one 26-monooxygenase. The enzyme can receive electrons from ferredoxin reductase in vitro, its natural electron donor is not known yet.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Reaction
cholest-4-en-3-one + 3 NADPH + 3 H+ + 3 O2 = (25R)-3-oxocholest-4-en-26-oate + 3 NADP+ + 4 H2O (overall reaction); (1a) cholest-4-en-3-one + NADPH + H+ + O2 = (25R)-26-hydroxycholest-4-en-3-one + NADP+ + H2O; (1b) (25R)-26-hydroxycholest-4-en-3-one + NADPH + H+ + O2 = (25R)-26-oxocholest-4-en-3-one + NADP+ + 2 H2O; (1c) (25R)-26-oxocholest-4-en-3-one + NADPH + H+ + O2 = (25R)-3-oxocholest-4-en-26-oate + NADP+ + H2O
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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