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Chemically modified Pseudomonas species Cholesterol Esterase

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Cat No.

DIA-281

Description

Hydrolase that splits fatty acids from sterols. Take advantage of the enhanced stability of this enzyme in liquid reagents. Rely on the proven diagnostic quality of this product.

Abbr

Chemically modified CE (Pseudomonas sp.)

Alias

Source

Pseudomonas species

Applications

Use Cholesterol Esterase, chemically modified in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase.

Appearance

Brownish lyophilizate

Activity

>10 U/mg lyophilizate; >100 U/mg protein

Contaminants

ATPase: <0.005 Catalase: <1.00 Glycerokinase: <0.001 Hexokinase: <0.005 "NADH oxidase": <0.001 Uricase: <0.005

Molecular Weight

~129 kD

Isoelectric point

4.5

pH Stability

6.0-6.5

Michaelis Constant

Cholesterol oleate: 7 x 10-5 mol/l

Specificity

Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.

Optimum pH

7.6

Activators

Detergents

Thermal stability

Below +20°C

Stability

At +2 to +8°C within specification range for 12 months. Store dry.

Inhibitors

Heavy metals such as Cu2+, Ag+, Zn2+

Synonyms

cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; sterol esterase; CE

"Cholesterol Esterase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1679	Native Candida Rugosa Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Candida Rugosa	Inquiry
DIA-133	Native Schizophyllum commune Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Schizophyllum commune	Inquiry
DIA-134	Native Pseudomonas sp. Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Pseudomonas sp.	Inquiry
DIA-135	Native Microorganism Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Microorganism	Inquiry

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