Description
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides; specifically, the hydrolysis of the 1,4-beta-D-glycosidic linkages in cellulose, hemicellulose, lichenin, and cereal beta-D-glucans. Cellulases break down the cellulose molecule into monosaccharides ("simple sugars") such as beta-glucose, or shorter polysaccharides and oligosaccharides. The name is also used for any naturally occurring mixture or complex of various such enzymes, that act serially or synergistically to decompose cellulosic material.
Abbr
Cellulase, Recombinant (Cellvibrio mixtus)
Source
Cellvibrio mixtus ATCC 12120
Form
Supplied in 35 mM HEPES buffer, pH 7.5, containing 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2 and 25 % (v/v) glycerol.
Enzyme Commission Number
EC 3.2.1.4
Molecular Weight
33835.5 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of glucose-reducing-sugar equivalents per minute from barley β-glucan in 50 mM Tris-HCl, pH 7.5, 5 mM CaCl2, at 37°C
Optimum pH
7.5 (stable from 6 – 8.5)
Optimum temperature
37°C (stable up to 45°C)
Storage
Store at -20°C (shipped at room temperature)
Synonyms
Cellulase, thermostable; 1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase; EC 3.2.1.4; Cellulase; endo-1,4-β-D-glucanase; β-1,4-glucanase; β-1,4-endoglucan hydrolase; celluase A; cellulosin AP; endoglucanase D; alkali cellulase; cellulase A 3; celludextrinase; 9.5 cellulase; avicelase; pancellase SS
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