Description
Caspase 1/Interleukin-1 converting enzyme is an enzyme that proteolytically cleaves other proteins, such as the precursor forms of the inflammatory cytokines interleukin 1β and interleukin 18, into active mature peptides. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages. Studies of a similar protein in mouse suggest a role in the pathogenesis of Huntington's disease. Alternative splicing of the gene results in five transcript variants encoding distinct isoforms. Recent studies implicated caspase 1 in promoting CD4 T-cell death and inflammation by HIV, two signature events that fuel HIV disease progression to AIDS.
Abbr
CASP1, Recombinant (Human)
Product Overview
Recombinant, human caspase-1 fused at the N-terminus to a His•Tag sequence and expressed in E. coli Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening.
Activity
>25,000 units/mg protein
Molecular Weight
10 kDa and 20 kDa
Unit Definition
One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-NA per min at 30°C, pH 7.4.
Storage
< -70°C; Avoid freeze/thaw
Buffer
In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Synonyms
CASP1; ICE; IL1BC; P45; Caspase 1; Interleukin-1; IL-1β Converting Enzyme
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