Description
The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are therefore classified as metalloenzymes.
Abbr
CA2, Recombinant (Bovine)
Applications
Carbonic anhydrase is used to create carbon dioxide capture systems and to research various purification techniques. Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome. Bovine carbonic anhydrase II (CA II), has been widely used as a model protein in the investigation of the protein folding process.
Appearance
Colorless clear liquid
Product Overview
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications. Carbonic anhydrases are metalloenzymes since most active sites contain zinc. Carbonic anhydrases are widely distributed in plant and animal tissues where they are involved in diverse physiological processes, such as photosynthesis, pH homeostasis, calcification, and bone resorption. The enzymes can be localized in the cytosol or mitochondria, be membrane bound with extracellular domains, or be secreted. Bovine carbonic anhydrase II (CA II) is a cytosolic, single-chain, approximately 29 kDa enzyme.
Form
Supplied as a solution in 20 mM Tris, pH 7.6, with 150 mM NaCl.
Enzyme Commission Number
EC 4.2.1.1
Activity
> 5,000 units/mg
Molecular Weight
29-31 kDa
Concentration
500-700 µg/ml
Unit Definition
One unit will decrease th pH of a 20 mM tris buffer from pH 8.3 to 6.3 per minute at 0 °C.
Stability
The product is stable for at least 2 years as supplied.
Storage
Store the product at –20 °C. After initial thawing, the enzyme should be refrozen at –20 °C in aliquots.
Synonyms
carbonic anhydrases; carbonate dehydratases; EC 4.2.1.1; anhydrase; carbonate anhydrase; carbonic acid anhydrase; carboxyanhydrase; carbonic anhydrase A; carbonate hydro-lyase
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