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carbamoyl-phosphate synthase (glutamine-hydrolysing)

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Cat No.

EXWM-5808

Description

The product carbamoyl phosphate is an intermediate in the biosynthesis of arginine and the pyrimidine nucleotides. The enzyme from Escherichia coli has three separate active sites, which are connected by a molecular tunnel that is almost 100 Å in length. The amidotransferase domain within the small subunit of the enzyme hydrolyses glutamine to ammonia via a thioester intermediate. The ammonia migrates through the interior of the protein, where it reacts with carboxyphosphate to produce the carbamate intermediate. The carboxyphosphate intermediate is formed by the phosphorylation of hydrogencarbonate by ATP at a site contained within the N-terminal half of the large subunit. The carbamate intermediate is transported through the interior of the protein to a second site within the C-terminal half of the large subunit, where it is phosphorylated by another ATP to yield the final product, carbamoyl phosphate. cf. EC 6.3.4.16, carbamoyl-phosphate synthase (ammonia).

Form

Liquid or lyophilized powder

Enzyme Commission Number

EC 6.3.5.5

CAS No.

37233-48-0

Storage

Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.

Synonyms

carbamoyl-phosphate synthetase (glutamine-hydrolysing); carbamyl phosphate synthetase (glutamine); carbamoylphosphate synthetase II; glutamine-dependent carbamyl phosphate synthetase; carbamoyl phosphate synthetase; CPS; carbon-dioxide:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); carA (gene name); carB (gene name); CAD (gene name); hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating)

Reaction

2 ATP + L-glutamine + hydrogencarbonate + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (overall reaction); (1a) L-glutamine + H2O = L-glutamate + NH3; (1b) ATP + hydrogencarbonate = ADP + carboxyphosphate; (1c) NH3 + carboxyphosphate = carbamate + phosphate; (1d) ATP + carbamate = ADP + carbamoyl phosphate;

Notes

This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.