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cAMP-dependent Protein Kinase catalytic subunit from Murine, Recombinant

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Cat No.

NATE-1888

Description

The catalytic subunit of cAMP-dependent Protein Kinase (PKA) is a serine/threonine protein kinase, which combines, in the absence of cAMP, with the regulatory subunit to form the inactive PKA holoenzyme. Since this is the free catalytic subunit alone, no cAMP is required for activation.

Source

E. coli

Species

Murine

Form

50 mM NaCl, 20 mM Tris-HCl (pH 7.5 at 25°C), 1 mM Na2 EDTA, 2 mM DTT and 50% glycerol.

Enzyme Commission Number

EC 2.7.11.11

Molecular Weight

38 kDa

Concentration

2,500,000 units/ml

Unit Definition

One unit is defined as the amount of PKA catalytic subunit required to catalyze the transfer of 1 pmol of phosphate to Kemptide, LRRASLG (100 µM) in 1 minute at 30°C in a total reaction volume of 25μL.

Storage

Store at -20° C

Synonyms

Protein Kinase A catalytic subunit; Protein kinase A; PKA; PKAC; cAMP-dependent protein kinase catalytic subunit; PRKAC

Specific Activity

5,000,000 units/mg

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1889	Native Bovine Protein Kinase A Catalytic Subunit	EC 2.7.11.11		Bovine Heart	Inquiry
NATE-1944	Native Bovine Protein Kinase A	EC 2.7.11.11	9026-43-1	Bovine heart	Inquiry
NATE-0571	Protein Kinase A catalytic subunit human, Recombinant			E. coli	Inquiry