Applications
SIGMA FAST BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium) Tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity. Common uses are in immunoblotting or dot blotting. It is less commonly used in immunohistochemistry. These tablets require no additional buffers or steps to prepare an active substrate solution.
Synonyms
BCIP/NBT Alkaline Phosphatase Substrate
Reconstitution
Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.
Substrates
Alkaline Phosphatase
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