Description
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Abbr
Apyrase, Recombinant (Potato)
Applications
Highly efficient degradation of ATP to AMP. Removal of deoxynucleotides in DNA pyrosequencing between cycles. Conversion of 5´ triphosphorylated RNA to ligatable monophosphorylated form that can be used for 5´ RNA adaptor ligation. Conversion of 5´ triphosphorylated RNA to 5´ exonuclease XRN-1 sensitive monophosphorylated RNA.
Product Overview
Apyrase (recombinant, E. coli) is a highly active ATP-diphosphohydrolase that catalyses the sequential hydrolysis of ATP to ADP and ADP to AMP releasing inorganic phosphate. It is a recombinant version of one of several isoforms of apyrase. It can also hydrolyse 5´ tri- and diphosphate ribonucleosides and deoxyribonuclesides to their respective 5´ monophosphates. Apyrase can catalyse the conversion of 5´ triphosphorylated RNA to 5´ monophosphorylated RNA by sequential removal of γ and β phosphates.
Form
50 mM NaCl, 20 mM MES (pH 6.5 25°C), 0.1 mM CaCl2, 1 mM DTT, 0.1% Tween-20 and 50% glycerol.
Concentration
500 units/ml
Unit Definition
One unit is defined as the amount of enzyme that catalyses the release of 1 μmol of inorganic phosphate from ATP (1 mM) in 1X Apyrase Reaction Buffer in 1 minute at 30°C in a total reaction of 50 μl.
Synonyms
ATP-diphosphatase; adenosine diphosphatase; ADPase; ATP diphosphohydrolase; apyrase; EC 3.6.1.5; 9000-95-7
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