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Alanine Dehydrogenase from Bacillus cereus, Recombinant
Cat No.
NATE-0044
Description
L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the geneRation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L-or D-cysteine.
Abbr
AlaDH, Recombinant (Bacillus cereus)
Alias
EC 1.4.1.1; 9029-06-5; AlaDH
Source
E. coli
Species
Bacillus cereus
Enzyme Commission Number
EC 1.4.1.1
Activity
> 350 units/ml
CAS No.
9029-06-5
Unit Definition
1 U corresponds to the amount of enzyme which converts 1 μmol L-alanine per minute at pH 10.0 and 30°C (NAD+ as cofactor).
Storage
−20°C
Synonyms
L-Alanine Dehydrogenase; Alanine dehydrogenase; EC 1.4.1.1; 9029-06-5; AlaDH; NAD+-linked alanine dehydrogenase; alpha-alanine dehydrogenase; NAD+-dependent alanine dehydrogenase; alanine oxidoreductase; NADH-dependent alanine dehydrogenase
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1801 | Alanine Dehydrogenase (Crude Enzyme) | EC 1.4.1.1 | 9029-06-5 | E. coli | Inquiry |
| NATE-1899 | Native Bacillus stearothermophilus Alanine Dehydrogenase | EC 1.4.1.1 | 9029-06-5 | Bacillus stearothermophilus | Inquiry |
| NATE-0043 | Native Bacillus subtilis L-Alanine Dehydrogenase | EC 1.4.1.1 | 9029-06-5 | Bacillus subtilis | Inquiry |
| EXWM-1442 | alanine dehydrogenase | EC 1.4.1.1 | 9029-06-5 | Inquiry |