Description
Ac-VDVAD-AFC is a fluorogenic substrate for caspase-2. It is similar to Ac-VDVAD-AMC but the AFC fluorophore has a greater Stokes' shift upon cleavage. VDVAD has been found to be a preferred cleavage site for caspase-2 (Ich-1L). Increases in Ac-VDVAD-AFC cleavage is correlated with losses in procaspase-2 (conversion to active caspase-2) in human neuroblastoma lines induced to apoptosis with C2-ceramide and NO.
Applications
A fluorogenic substrate for caspase-2
Synonyms
Caspase Substrate (AFC); Ac-Val-Asp-Val-Ala-Asp-AFC
Molecular Formula
C33H41F3N6O12
Solubility
Soluble in DMSO (20 mM), and DMF (20 mM).
Substrates
Caspase-6; Cysteine proteases
Download Datasheet