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T4 β-glucosyltransferase, Recombinant
Cat No.
NATE-0773
Description
In enzymology, a DNA beta-glucosyltransferase is an enzyme that catalyzes the chemical reaction in which a beta-D-glucosyl residue is transferred from UDP-glucose to an hydroxymethylcytosine residue in DNA. It is analogous to the enzyme DNA alpha-glucosyltransferase. This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases.
Abbr
β-glucosyltransferase, Recombinant
Source
E. coli
Applications
Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.
Product Overview
T4-phage beta-glucosyltransferase, also known as UDP glucose-DNA ?-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351 (end) with C-terminal His-tag, MW= 41.6 kDa, expressed in E. coli.
Form
aqueous solution, Formulated in 200 mM imidazole and 20% glycerol.
Molecular Weight
mol wt 41.6 kDa
Purity
> 83% (SDS-PAGE)
Storage
−70°C
Synonyms
T4 Beta-glucosyltransferase; DNA beta-glucosyltransferase; T4-HMC-beta-glucosyl transferase; T4-beta-glucosyl transferase; T4 phage beta-glucosyltransferase; UDP glucose-DNA beta-glucosyltransferase; uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase
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