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Serratia marcescens nuclease (SMnuclease), Recombinant
Cat No.
EXWM-3708
Description
SMnuclease is the only known nuclease that can hydrolyze all types of nucleic acids, including single-stranded, double-stranded, linear, and circular DNA or RNA. The DNA hydrolysis rate of SMnuclease is 34 times that of DNaseI, and 4 times that of Staphylococcus aureus nuclease. SMnuclease can react under a very wide range of conditions (such as 6 M Urea, 0.1 M Guanidine HCl, 0.4% Triton X-100, 0.1% SDS, 1 mM EDTA, 1 mM PMSF) and is widely used to remove nucleic acids from biological products.
Species
Serratia marcescens
Form
Colorless clear liquid
Enzyme Commission Number
EC 3.1.30.2
Activity
≥ 1.5×10^6 U/mg
CAS No.
9025-65-4
Molecular Weight
28.0 kDa
Purity
>90% by SDS-PAGE
pH Stability
pH 6-10
Unit Definition
The amount of enzyme that reduced the value of ΔA260 by 1.0 (equivalent to the complete digestion of 37 μg Salmon DNA) within 30 minutes under the reaction conditions of 37°C and pH 8.0 is defined as an active unit.
Optimum pH
8
Optimum temperature
37°C
Storage
The product is stored at -20°C. The shelf life is 24 months. The product is stable under the condition of cold and airtight storage, and the activity is not significantly attenuated.
Reaction
Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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