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scytalidopepsin B
Cat No.
EXWM-4270
Description
A second endopeptidase from Scytalidium lignicolum (see scytalidopepsin A) that is insensitive to pepstatin and methyl 2-diazoacetamidohexanoate. 1,2-Epoxy-3-(p-nitrophenoxy)propane reacts with Glu53, which replaces one of the aspartic residues at the active centre. One of the smallest aspartic endopeptidases active as the monomer, with Mr 22,000. Similarly inhibitor-resistant endopeptidases are found in the basidiomycetes Lentinus edodes and Ganoderma lucidum, and in Polyporus tulipiferae, a second endopeptidase distinct from polyporopepsin, but these are of typical aspartic endopeptidase size, Mr about 36,000. Type example of peptidase family G1.
Form
Liquid or lyophilized powder
Enzyme Commission Number
CAS No.
104781-89-7
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
Scytalidium aspartic proteinase B; Ganoderma lucidum carboxyl proteinase; Ganoderma lucidum aspartic proteinase; Scytalidium lignicolum aspartic proteinase B; SLB
Reaction
Hydrolysis of proteins with broad specificity, cleaving Phe24┼Phe, but not Leu15-Tyr and Phe25-Tyr in the B chain of insulin
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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