Description
Native Saccharomyces cerevisiae Hexokinase for research on glucose metabolism and enzymatic mechanisms. Ideal for biochemistry and molecular biology studies. Creative Enzymes ensures reliable products.
Abbr
Hexokinase, Native (Saccharomyces cerevisiae)
Source
Saccharomyces cerevisiae
Form
Type I, Lyophilized powder containing phosphate/Citrate pH approx. 7.0; Type II, Type III, Lyophilized powder containing approx. 15% sodium Citrate.
Enzyme Commission Number
EC 2.7.1.1
Activity
Type I, > 350 units/mg protein; Type II, > 25 units/mg protein (biuret); Type III, > 130 units/mg protein (biuret).
Molecular Weight
~ 54 kDa (monomer); ~110 kDa (dimer)
Unit Definition
One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25°C, unless otherwise indicated below.
Activators
Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.
Inhibitors
sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
Warnings
Predominantly the PII isoform, produced from an overexpressing yeast strain.
Function
ATP binding; catalytic activity; hexokinase activity; ATP binding; catalytic activity; hexokinase activity
Synonyms
hexokinase type IV glucokinase; hexokinase D; hexokinase type IV; hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; hexokinase; ATP:D-hexose 6-phosphotransferase; EC 2.7.1.1; 9001-51-8