Products
Online Inquiry
Our Products Cannot Be Used As Medicines Directly For Personal Use.
Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.
Native Penicillium citrinum Nuclease P1
Cat No.
NATE-0491
Description
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea.
Abbr
Nuclease P1, Native (Penicillium citrinum)
Alias
Nuclease P1; P1 nuclease
Source
Penicillium citrinum
Applications
Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA. Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts. The enzyme has an optimal temperature of approximately 70 oc, but for a long incubation, a temperature below 60 oc is more suitable. It is stable in the pH range of 5-8.
Package
vial of > 250 units (using RNA substrate)
Form
lyophilized powder
Enzyme Commission Number
EC 3.1.30.1
Activity
> 200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase)
CAS No.
54576-84-0
Molecular Weight
42-50 kDa
Unit Definition
3'-5'-Phosphodiesterase: One unit will liberate 1.0 μmole of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3'-Nucleotidase: One unit will hydrolyze 1.0 μmole of orthophosphate from 3'-AMP per min at pH 7.2 at 37°C.
Storage
2-8°C
Synonyms
Endonuclease P1; EC 3.1.30.1; 54576-84-0; Nuclease P1; P1 nuclease
Download
Download Datasheet"Nuclease" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0492 | Native Aspergillus oryzae Nuclease S1 | EC 3.1.30.1 | 37288-25-8 | Aspergillus oryzae | Inquiry |
| EXWM-3707 | Aspergillus nuclease S1 | EC 3.1.30.1 | 37288-25-8 | Inquiry |