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Native Bovine Enterokinase
Cat No.
NATE-0224
Description
Enteropeptidase (also called enterokinase) is an enzyme produced by cells of the duodenum and involved in human and animal digestion. It is secreted from intestinal glands (the crypts of Lieberkühn) following the entry of ingested food passing from the stomach. Enteropeptidase converts trypsinogen (a zymogen) into its active form trypsin, resulting in the subsequent activation of pancreatic digestive enzymes. Absence of enteropeptidase results in intestinal digestion impairment.
Abbr
Enterokinase, Native (Bovine)
Source
Bovine intestine
Species
Bovine
Applications
Conformation of FLAG peptide removal can be determinied by dot blot assay and SDS-PAGE analysis using nitrocellulose. Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags. Removes FLAG peptide from N-terminal and Met-N-terminal fusion proteins.
Product Overview
Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.
Form
powder
Enzyme Commission Number
EC 3.4.21.9
Activity
Type I, > 20 units/mg protein
CAS No.
9014-74-8
Molecular Weight
150 kDa (consisting of 115kDa and 35kDa subunits.)
Storage
−20°C
Function
scavenger receptor activity; serine-type endopeptidase activity
Synonyms
enterokinase; enteropeptidase; EC 3.4.21.9; 9014-74-8
Download
Download Datasheet"Enterokinase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0225 | Native Porcine Enterokinase | EC 3.4.21.9 | 9014-74-8 | Porcine intestine | Inquiry |
| NATE-0226 | Enterokinase from bovine intestine, Recombinant | EC 3.4.21.9 | 9014-74-8 | E. coli | Inquiry |
| NATE-0227 | Enterokinase from Human, Recombinant | EC 3.4.21.9 | 9014-74-8 | CHO cells | Inquiry |
| NATE-0872 | Native Calf Enterokinase | EC 3.4.21.9 | 9014-74-8 | Calf intestine | Inquiry |