Products
Online Inquiry
Our Products Cannot Be Used As Medicines Directly For Personal Use.
Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.
Native Bovine β(1-3,4)-Galactosidase
Cat No.
NATE-0973
Description
Hydrolyzes non-reducing terminal galactose β(1-3) and β(1-4) linkages. Can be used in conjunction with other β-galactosidases for exoglycosidase sequencing.
Abbr
GLB, Native (Bovine)
Alias
β-gal; GLB
Source
Bovine testis
Species
Bovine
Applications
The enzyme has applications in the analysis of a wide variety of glycoconjugates. It is particularly useful for ensuring the complete removal of β(1-3) and β(1-4)-linked non-reducing terminal galactose residues of oligosaccharides. Gal β(1-6) GlcNAc is hydrolyzed more slowly, however this linkage is not normally encountered in native complex glycans. This activity towards β(1-3) and β(1-4)-linked galactose contrasts with that of our other β-galactosidases which exhibit a preference for Gal β(1-4), and cleave the Gal β(1-3) linkage relatively slowly, if at all. Used in conjunction, these enzymes provide a powerful means to determine linkage positions of non-reducing β galactose residues.
Form
20 mM sodium Citrate phosphate, 150 mM NaCl (pH 4.0)
Molecular Weight
~68 kD
Unit Definition
One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP-β-D-galactopyranoside per minute at pH 4.0 and 37°C.
Optimum pH
4
Buffer
5X concentrated buffer which when diluted gives 100 mM sodium Citrate/phosphate pH 4.0.
Synonyms
β-galactosidase; beta-gal; β-gal; lactase; β-lactosidase; maxilact; hydrolact; β-D-lactosidase; lactozym; trilactase; β-D-galactanase; oryzatym; sumiklat; β-D-galactoside galactohydrolase
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1745 | Native Bacillus circulans β-Galactosidase | EC 3.2.1.23 | 9031-110-2 | Bacillus circulans | Inquiry |
| NATE-0295 | Native Bovine β-Galactosidase | EC 3.2.1.23 | 9031-11-2 | Bovine testes | Inquiry |
| DIA-189 | Native Escherichia coli β-Galactosidase | EC 3.2.1.23 | 9031-11-2 | Escherichia coli | Inquiry |
| DIA-220 | Native Aspergillus oryzae β-Galactosidase | EC 3.2.1.23 | 9031-11-2 | Aspergillus oryzae | Inquiry |